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Meigh, L. et al. CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits.
Protein protein interactions between subunits of bacterial NRPSs are essential for proper template formation.
The distance between subunits at the opening is sufficiently wide (12 Å) to accommodate single-stranded RNA.
Analysis of ATP substrate inhibition kinetics on ATP hydrolysis in hexameric PspF1 275 indicates negative homotropic effects between subunits.
This study charts the landscape of multi-domain protein structures that form intertwined homodimers by exchanging structural domains between subunits.
After mixing both proteins, there was a time dependent increase in the 530 nm emission of the acceptor upon excitation at 436 nm, suggesting that exchange between subunits of tetrameric complexes was taking place.
The data reveals that helix D stabilizes CaM binding, promoting trans-binding (CaM embracing neighboring subunits), and they suggest that the ABCD domain can be exchanged between subunits of the tetramer.
Interestingly, these cryo-EM images reveal that the helix D region is very flexible47, an observation in line with the exchange between subunits observed here for the isolated C-terminal domain.
In addition, due to the absence of physical bonds between both ABCG2 monomers, we also assessed their relative free energy of binding between subunits with g_mmpbsa added with the implicit membrane correction.
Rational design was applied to thermophilic TA from Thermotoga maritima to improve thermal stability by the incorporation of salt and disulfide bridges between subunits in the functional tetramer.
One of the pockets is limited in size while the other is of considerable dimensions and protrudes along the interfacial cleft between subunits.
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