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Since the flow was laminar, a sharp interface between streams was maintained.
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Three-dimensional reconstruction of HNK1- (glycan epitope labelling NCC) and NFM- (neurofilament medium chain; neuroblasts) stained chick embryos revealed that early hindbrain NCC streams were maintained, forming robust structures between hindbrain and pharyngeal arches (Fig. 1A,B; supplementary material Movie 1).
The jar was placed in an oil bath preheated to 333 K, and the nitrogen stream was maintained for 3 h.
The flow rate of odorant streams (vanilla scent) was maintained at level of 50 mL/min, and a variable air flow dilution of 5% was produced.
The gap between the plates was maintained at 3 mm, providing channels through which soluble gas pollutants in the upward gas stream were absorbed by the downward scrubbing liquid film on the surfaces.
The total carbon mass flow rate was maintained constant when the fraction of DME in the fuel stream was varied.
None of the stream pairs were connected by stream flow at the sampling locations, thus larval dispersal (drift, crawling) between streams was unlikely, whereas adult dispersal (flying) between streams was possible.
In this catchment, a no-spray boundary of approximately 30 m was maintained around the stream margins (Fig. 1).
The obscuration range was maintained between 5%and20%0%.
Temperature was maintained between 2°C 8°C.
Body temperature was maintained using a temperature-controlled air stream around the body of the mouse.
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