Differences between murine strains were analyzed using ANOVA with Fisher's PLSD test for pair-wise comparison (statview 4; Abacus Concept, INC., Berkeley, CA).
Distribution differences in the functional categories of CDSs assigned according to COG between PCN033 and PCN061 strains were analyzed using Pearson's χ tests.
b OrthoANI values and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) trees between strain GFP-2 and closely related strains were analyzed by Orthologous Average Nucleotide Identity Tool (OAT).
OrthoANI values and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) trees between strain GFP-2 and closely related strains were analyzed by Orthologous Average Nucleotide Identity Tool (OAT) (Lee et al. 2016).
The differences in biofilm production between imipenem-selected mutants and the parental strains were analyzed using Student's t-test, as appropriate.
The polymorphic sites between the genomes of the W. ceti strains were analyzed with the whole-genome multilocus sequence typing (wgMLST) methodology using the gene-by-gene approach in the BIGSdb software, installed in a local server [ 24, 25].
In the present study, four gene loci 16S rRNA, ITS-L, cpcBA-IGS and rpoC1, sequenced from many Chinese strains (44 Cylindrospermopsis strains and 16 Raphidiopsis strains), were analyzed to infer the phylogenetic relationship between the two genera.
All 142 strains were analyzed by MLST.
Mutant strains were analyzed microscopically at different temperatures.
Three independent transgenic strains were analyzed.
