Lung transplantation was performed in 48 mice, applying major or minor histocompatibility mismatches between strains for the induction of CAF.
Significant differences were observed between strains for basidiome number and weight, while no significant differentiation for quantitative morphological traits was observed between geographical origins and taxonomic groups.
In this context, generalized cross-immunity acts to considerably increase the competition between strains for susceptible hosts (as well as to reduce the overall infected population size).
The composition of several gene families also differs between strains; for instance, S288C includes only one (SUC2 ) of an eight-member invertase gene family (Carlson and Botstein 1983).
Quantitative RT-PCR was used to confirm the expression differences between strains for a subset of 41 differentially expressed and similarly expressed genes.
However, it showed some variability between strains; for example, the T4SS genes were present in USDA 110T but were absent in CPAC 7 and USDA 6T.
Pairwise distance between strains (for NGS and clonal analysis, this was calculated between the most similar pretreatment and posttreatment strains) was significantly higher using direct sequencing than NGS (mean 0.221 versus 0.026, respectively; P = 0.0002).
PCR results were first analysed by gel electrophoresis, which detected no difference between strains for VNTR5 or VNTR19, with all profiles identical, including those of the PG2 type strain and of 5632.
We investigated whether there was differential fat accumulation in mice fed Western versus regular chow and whether there were genotypic differences for fat accumulation between strains for each diet.
46 The utility of strain typing is supported by the development of large web-based national and international strain registries which allow comparisons to be made between strains, for local contact tracing, and also for wider epidemiologic investigations, for example the MIRU-VNTRplus tool.
