Between the eighth and third days preceding the start of experiment, we scored grooming behavior of birds (5 days in total).
Adult male mice of the alcohol-preferring C57BL/6J strain, weighing between 20 and 22 g at the start of experiments were also investigated.
Adult male mice of the alcohol-preferring C57BL/6J strain, weighing between 18 and 21 g at the start of experiments, were also studied.
Adult normal male Wistar rats weighing between 150 and 170 g at the start of experiments were purchased from accredited animal suppliers and were acclimatized to our standard UK Home Office-approved housing conditions (21 ± 2°C, relative humidity of 55 ± 10% and a 12 h/12 h light: dark cycle) for at least one week before experiments.
Adult normal male Wistar rats weighing between 150 and 170 g at the start of experiments were purchased from accredited animal suppliers and were acclimatized to our standard UK Home Office-approved housing conditions (21 ± 2°C, relative humidity of 55 ± 10% and a 12 h/12 h light:dark cycle) for at least 1 week before experiments.
Fresh 35°C culture medium was used at the start of experiments and when changing medium between patterned stimulation and whole-dish probing experiments.
TTTR is a time-correlated single-photon counting (TCSPC) technique capable of recording all time-related information for every detected photon, including the relative time between the excitation pulse and photon emission as well as the absolute time between the start of the experiment and the photon emission.
As for Ag-EN dissolution, it took place rather quickly, with [Ag+]T reaching a maximum concentration in approximately 1 h (the time lag between the start of this experiment and the first sampling for ultrafiltration) despite the fact that surface coating was expected to slow down the dissolution of nanoparticles [17], [18].
Looking at the seven unknown families collected at the farm, at three stages of the experiment; infection, sampling and after termination of the EB trial, the distribution of family representation in the overall population changed clearly between the start of the experiment and after termination of the EB trial.
The interval between the start of the experiment (red LED) and the green LED lit time was pseudorandomly programmed in a CPU board (6 external interrupts control, 40 MHz CPU on R-Engine-A board time counters of 0.6 μs time resolution from Tern Inc).
