The G Ic data is highly consistent for each specimen between methods, and between specimens of each method (small values of standard deviation).
There was no significant difference in HCMV DNA copy number between urinary and respiratory specimens for each patient, suggesting that urine is a suitable sample for pediatric acute pulmonary HCMV infection.
Genetic distances between specimens were calculated for each intraspecies (S), intragenus (G) and intrafamily (F) with the 'Distance Summary' command implemented by BOLD.
Changes in proliferative indices and receptor expression levels between core biopsy specimens and operative specimen for each subgroup were compared using paired sample t-tests.
There was concordance between paired specimens for at least one species in 90 episodes (76.9%); amongst these there were 39 (42.4%) episodes in which discordance between specimen pairs for a different species was also present.
Concordance between both specimens for all etiologic agents was 28%, for Staphylococcus aureus 38%, and for organisms other than S. aureus 19%.
We computed the percentage change in the proportion of positive specimens (PPS) for each virus between each month of 2003 and the mean PPS in the same month of the preceding 5 years (1998 2002), which served as the reference period.
In addition, different samples are prepared and measured at varying times, but high tissue growth deviation between specimens and the need for monitoring tissue growth and scaffold degradation at different times requires large sample numbers for statistical analysis.
If ribosomal DNA copies within a specimen are more diverse than between specimens, phylogenetic analyses using one representative sequence for each sampled specimen may not accurately represent relationships by descent.
As a consequence of the differences in HPV detection by specimen type, the concordance between the two specimens for HPV detection in any HPV category was only fair, with kappa values around 0.50.
