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This is especially true where the channel widths and distance between sources of activator are reduced, meaning that the circuit definition is functionally reduced.
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The application of activator at both inputs resulted in signal splitting and two outputs (i.e., the P. Polycephalum moved toward both sources of activator).
There is noticeable segregation of the culture to different localized areas of the dish corresponding to the sources of activator.
This is presumably because there is already activator within the circuit so the termination points of the circuit (at least in the short/medium term) are sources of activator at the various inputs.
The result is the propagation of P. polycephalum up the input channel, where it splits and moves toward both sources of activator in the output channels.
There is obviously some time dependence to this effect and the P. Polycephalum would eventually explore the whole environment once the sources of activator are depleted.
In addition to exogenous DNAs, self-DNAs are also sources of activators that initiate type I interferon production through cGAS.
So, S80 fraction prepared from Nonabokra was used as the source of activator (kinase/phosphatase/any other organic compound).
Thus the signal is routed through 3 outputs all of which were sources of the activator chemical farnesene.
It was found that P. polycephalum would adjust its spatial configuration to span four, three and two sources of the activator (in various locations).
In this case the P. polycephalum correctly implements the central junction (which has two sources of activation) implementing signal splitting, but leaves the confines of the channel and moves toward the activator source external to the channels of the circuit.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com