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Different site models [24] that allow ω to vary between sites were tested (models M1a, M2a, M3, M7, M8).
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The significance of LD between sites was tested by Fisher's exact test.
Therefore, the distinctions between the following sites were tested: upstream, where water is free from anthropogenic activities, such as industries, domestic sewage and agricultural contaminations, and downstream, where the effluent disposal of all industries and the municipal sewage are discharged.
Data within and between the clinical trial sites were tested for homogeneity of variance and normalcy.
Significant associations between mtDNA and nDNA sites were tested using linkage disequilibrium analysis based on the D parameter [ 98].
Differences in activity between the two oxygen treatments and sites were tested using a 2-way ANOVA (stats-package).
When these 11 ANOVA-significant sites were tested for differences between the fertile controls and the two ART groups (infertile and donor oocyte) by Tukey's post hoc HSD test, 7 of the 11 sites differed between the donor oocyte ART group and the fertile control groups and 2 of the sites differed between the infertile group and the fertile controls.
10 sites were tested for each sample.
The different effect of the QTL between the two sites was tested according to Rae et al. [ 60].
Differences in categorical variables between the two study sites are tested using a Chi-squared test of association, while differences in continuous variables between study sites are compared using the Wilcoxon Rank sum test.
In order to test whether there is recombination within cyt b gene, linkage disequilibrium (LD) test was conducted using DnaSP 4.10 [ 73] and the average degree of LD, or non-random association between nucleotide variants at different polymorphic sites was tested using ZZ statistics [ 74], which could be used for detecting intragenic recombination with the null hypothesis no recombination.
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