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Sequence alignment was conducted between the sequencing sequence and the reference genome with the software of Burrows-Wheeler Aligner (BWA) [ 28].
With R 2 → 1, within-sequence variance dominates between-sequence variance, and all sequences escape the influence of starting points and traverse all target distributions.
π Nucleotide diversity – the average number of nucleotide differences per site between two sequences * Sequence variation between mcyB1 and mcyC sequences # Sequence variation is 27 30% between Anabaena and Microcystis, 30 32% between Anabaena and Planktothrix, 32 34% between Microcystis and Planktothrix.
There was a negative correlation between sequencing coverage and site specific sequencing error rate (rS = −0.083, df = 15,786, p < < 0.001).
By fusing the result of pagerank and structure similarity, we can obtain the similarity score between sequence and sequence by (7).
Similarity percentages between sequences and reference sequences were assessed with the SimPlot program version 3.5.1.
Information was collected for both homozygous high quality discrepancies between sequences and heterozygous peaks within sequences.
These differences are polymorphic sites between the genome sequencing and the EST sequencing individuals.
As a result, a discontinuity can be seen due to the mismatch of final and initial joint positions between sequence 1 and sequence 2 1.
We also used notation Seq (x:y) meaning alignment scores between sequence x, and sequence y, and the scores were applied further for analysis.
These observations did not differentiate between sequence-dependent and sequence-independent uORFs.
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