Exact(2)
Regardless of high or low target region GC content, there was a negative correlation between sequencing coverage and extreme GC content.
There was a negative correlation between sequencing coverage and site specific sequencing error rate (rS = −0.083, df = 15,786, p < < 0.001).
Similar(58)
However, the improvement in performance is to arrive at a good balance between sequence coverage and computational time.
There is a strong relationship between sequence coverage and proportion of successful SNP genotype calls across individuals.
The mouse Xm brain and XX brain libraries represent the libraries sequenced with the lowest depth of coverage in our RNA-seq data collection, suggesting an interaction between sequence coverage and the analysis of global gene expression (discussed below).
To investigate the relationship between sequence coverage and number of variants discovered, aligned reads from both WGA BAM files were downsampled to different levels average target coverage using the Picard v1.50 program DownsampleSam.
Sequence coverage across the molecule was assessed in terms of a) redundant and bi-directional coverage, b) the degree of additional manual re-processing that would be required to develop complete replicate coverage, and c) the correlation between sequence coverage and sequence distance from the revised Cambridge Reference Sequence (rCRS) [ 22].
Sequencing, coverage, and error rates.
Therefore, high-throughput sequencing of amplified partial 16S rDNA sequences of a bacterial community currently provides the best compromise between sequence coverage, analytical speed and experimental costs.
The algorithm is not able to assemble short sequence repeats to large contigs as it is not possible with short read lengths to distinguish between high sequence coverage and large domains of short sequence repeats.
We focused on the relationship between average sequence coverage and sequence divergence in different chipmunk species libraries when using I. tridecemlineatus (divergent) and T. alpinus (closely related) genomes to design arrays.
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