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Focusing analyses on gene-coding regions provides a plausible trade-off between sequencing cost and discovery of informative variants, and may be an attractive study design before genome-wide sequencing study becomes widely available [14], [30], [46], [47], [48].
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Sampling genomes with Fosmid vectors and sequencing of pooled Fosmid libraries on the Illumina platform for massive parallel sequencing is a novel and promising approach to optimizing the trade-off between sequencing costs and assembly quality.
The 33 nt length (36 nt minus the 3 nt barcode) used in the study here represents an effective trade-off between sequencing costs (time and money) and read uniqueness.
Sequence capture, which utilizes probes to enrich target sequences (e.g. Bi et al. 2013) (Box 1), offers a compromise between minimizing sequencing costs and maximizing sequence information from target regions of the study organism.
Striking an ideal balance between assembly error types, rates and sequencing cost is an exercise left for users of our software.
This technology created a balance between higher genotyping accuracy and relatively lower sequencing cost.
Among others, the advantage of SLAF-seq lies in that it can create a balance between higher genotyping accuracy and relatively lower sequencing cost [ 29, 30].
Fortunately, due to the low sequencing cost, small RNA and RNA sequencing are routinely processed and we may be able to infer regulation relationships between miRNAs and mRNAs more accurately by using sequencing data.
One drawback of WGS is that, due to high sequencing cost, the sequencing coverage of WGS is typically between 30× and 60×, lower than that of other sequencing technologies, such as whole exome sequencing and targeted sequencing.
Each sequencing cost $100,000.
The sequencing cost $50,000.
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