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All comparisons between sequences were made using the program L-Align from ExPASy Molecular Biology Server.
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In one sample V1-V2 fragmenthehe nucleotide differences were too large between clones, two consensus sequences were made and separately analysed.
The genome sequence is making clear why.
Sequence alignments were made with ClustalW [84].
The sequencing reactions were made with ABI PRISM Big Dye Terminator Cycle Sequencing Ready Reaction Kit Applied Biosystemss).
Comparisons were made between sequences and the consensus derived from the number of sequences in column 3. Ae. aegypti sequences were obtained by database search using a query that spans the same sequence amplified by PCR (see Figure 1).
For seven of the eight HD-20 filtration panel members (excluding HolKor 2557 for which genotype data was not available), comparisons were made between sequence-derived genotype calls and those of 9,186 di-allelic PHR SNPs.
Small breaks were made between individual sequences, so as to allow patients to clear the throat of mucous secretions, to cough and to swallow between the sequences.
More detailed comparisons were made between the peptide sequences obtained from the protein spots and the deduced amino acid sequences of the 11 active LMW-GS genes isolated in this work (Table S5).
Comparisons were made between these two crab sequences and the E. superba sequences across a common region of 128 amino acids.
Alignments between reference genome sequence and SNPs amplified fragments were made using Sequencher software, in order to confirm these SNPs.
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