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CCL3, CCL4 and CCL5 mRNA levels, CCL4+ cell numbers in MLN and percentages of CCL4+ cells in peripheral blood CD4+ and CD8+ T cell populations were compared between selected time points of SHIVSF162P4-infected macaques by Student's t test and a p<0.05 was considered statistically significant.
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While previous studies have demonstrated an association between fluid overload at selected time points and death, ours is only the second study after that of Bellomo et al. [ 4] to demonstrate the relationship between positive fluid balance on RRT and mortality in critically ill adults, and suggests that fluid management during RRT is an important target for future clinical trials.
The point estimates and 95% point-wise confidence intervals (CIs) of cumulative incidence rates at selected time points between April and June of 2009 were calculated using generalized estimating equation (GEE) with GENMOD procedure in the SAS statistical package version 9.2 (SAS Institute Inc., Cary, NC) to account for correlated data within household.
Bacterial dissemination and lymphocyte activation were concomitantly analyzed at selected time points.
At selected time points, three mice in each group were anaesthetized and sacrificed.
At selected time points following MPTP intoxication, mice were sacrificed and brains processed for subsequent analyses.
Survival at selected time points was extrapolated from graphs.
For selected time points serum lipoproteins were separated by Fast Performance Liquid Chromatography FPLCC).
Cells were then cultured at 37°C and harvested at selected time points for analysis.
The abdominal tumor burden (signal intensity) was then recorded at selected time points.
At selected time points, cells were harvested for flow cytometry to analyze cell cycle distribution.
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