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The data consisted of nine-frame scan with 30-s acquisition time for each frame, and the interval between scans was set at 122 s.
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The number of scans was set to 6k-12k.
The time interval between the start of each set of scan was set at 1 minute (60 seconds) so that every individual scan for different heights (1st, 2nd, 3rd or 4th) in subsequent cycles started exactly at an interval of 60 seconds.
The scan was set to an ultra high-resolution scan mode using the manufacturer's software.
The heating and cooling rates for thermal scanning are set to be 20 K min−1.
The borderline between upper and lower scan range was set at approximately 2 cm below the carina.
The scan range was set between m/ z 200 and 1200 with a 10-ms ion accumulation time in MS data-dependent mode.
The scan size was set to 0 nm and scan rate was set to 0.1Hz.
The scan rate was set at 0.7 Hz, and the scanning size was 10 × 10 μm.
The scanning wavelength was 200 270 nm and the scanning rate was set at 50 nm/min.
The scanning resolution was set to 5 µm and the scan region was adjusted to 61×21.6 mm.
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