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The interaction between sampling method and a species' behaviour was recognised over 30 years ago [18].
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We next developed a method to detect relative AI between samples (Methods) and applied it to the three paired samples.
The relative abundance of bacterial or fungal species and overall diversity did not differ between sampling methods.
Previous studies have shown a limited concordance between tag sampling methods and microarrays (van Ruissen et al. 2005; Haverty et al. 2004; Kavsan et al. 2007).
However, the sensitivity and specificity of these tests may vary between sampling methods, or reference populations, even when the same tests are used in different species.
I will also compare numbers of Red-spotted newts between sampling methods.
Methodological heterogeneity exists between sampling methods used to assess TSR.
Agreement between sampling methods was evaluated by the following statistical tests: 'Kappa', 'The adjusted rand', McNemar's test for marginal symmetry, Proportion of agreement P0, P+, P-, and Odds Ratio.
We weighted percentages and means to allow for design effects introduced by variations between countries in sampling methods and household size (further details provided in online supplement; Supplementary Table S2 shows the sources used to apply non-representativeness weights and variables used in weighting).
Subsequent analysis of developmental and adult scaling of cerebra and medulla revealed significant differences in intercepts (Table 2); note however that we cannot exclude the possibility that this intercept difference is due to systematic differences in sampling method between adult and developing datasets.
Depending on the data set and sampling method, it took between 80 to 560 annotated samples to achieve mean average and root mean squared error below 0.01.
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