Cover and coral recruitment data were analyzed for differences between sampling dates and sites using a two-way analysis of variance, with dates and localities as fixed factors and quadrats as replicates.
However, rooting the tree on the oldest outbreak reveals a strong correlation between sample date and root-to-tip distance, with a substitution rate of 8 × 10−4 per site per year (Fig. 2B and fig. S7) (13).
This could possibly be due to a relatively short period of time passing between sample date and analysis, as MMP-9 has been shown to degrade even at low temperature [31].
Using the Path-O-Gen software (version 1.4, available from http://tree.bio.ed.ac.uk/software/pathogen/), we did not find a positive correlation between the sampling date and root-to-tip divergence for either subtype.
Of the 29 households at which poultry died, the median interval between the sampling date and death of the last bird was shorter among the 10 households for which environmental samples were positive (median days 0.5 vs. 16, p = 0.005) compared with 19 households with environmental samples with negative results.
This included the age of the patients, the PI-RADS and Gleason score, PSA blood level, and the viable tumor volume as well as the time difference between PSMA-PET and MRI, between Gleason score and PSMA-PET, between PSA blood level sampling date and PSMA-PET.
As expected, soil OC and TN contents were similar between sampling dates in May and September (P > 0.05) while active C, NH4+-N, WEOC and WEN contents were higher (P < 0.05) in May than September.
Microarray samples were taken from similar plants at 40 days after sowing ns not significant *p ≤ 0.05 (significant); **p ≤ 0.01 (significant) based on t tests between means of genotypes within sampling dates and P levels.
Daily N2O flux rates for dates between sampling dates were calculated using linear interpolation, and annual cumulative N2O emissions were calculated as the sum of all daily flux rates for the vegetation period of each crop and the entire investigation periods during March 2014 August 2015.
To test if differences in clonal composition increase with the time interval between samples, the clonal turnover rate was calculated between sampling dates (for all possible pairwise comparisons), per taxon and lake, by computing complements of the Morisita-Horn index (1-MH [ 48]; see also [ 49]) using the program SPADE [ 50] and 10 bootstrap replications.
