We used a variable number of seeds and maternal plants reflecting between-population variation in number of maternal trees available for sampling and seed viability.
To minimize variations between samples, the seed and non-seed components including glumes, lemmas and paleas were harvested from the middle region of each spike (6 10 spikelets; 2 3 florets per spikelet).
There were no differences between islands in the between-sample variation in seed density (χ = 4.69, df = 2, P > 0.05, R m 2 = 0.15, R c 2 = 0.20 ).
For the SEED annotations, similarities percentages breakdown, SIMPER [47] was used to determine which functional categories and SEED subsystems contributed most to differences between groups of samples (grouped by day/night and season).
B73 sample was then compared to inbred line B73 from Scuola Superiore Sant'Anna (B73-SSA, i.e., the parental line of the original B73 × H99 cross) in order to determine the level of divergence between samples from different seed stocks.
For each drug combination, triplicate samples were seeded and analysed.
Results show a trade-off between nearby sampling (many seeds, no long-distance dispersal measured) and distant sampling (few seeds, but long-distance dispersal measured).
He analyzed soil samples and developed seed mix, which was then machine-drilled into the soil rather than broadcast.
We also describe variation of genomic traits across our sample and highlight some differences and similarities in repeat structure between ferns and seed plants.
Dormancy classifications are different between bud and seed.
