Specimens were scanned by micro-computed tomography, which showed no material or microstructural differences between samples, and processed for histological analyses and damage quantification.
Diverse experimental protocols have demonstrated that the results of PCR-based methylation analyses are variable between experimental samples, and the analyses fluctuate according to the PCR conditions even with examining the same tissue DNA [ 39].
While the exact "significant" gene lists varied slightly, much of this difference is probably due to differences in sample treatment and analyses between the two array types, which determine the probability of detecting differences.
A sample holder was fitted with 3D stages so that different samples could be analysed without the need for opening the chamber in between sample analyses.
To perform a fair comparison between samples, all subsequent analyses were performed according to the normalized data (Zhou et al. 2016).
Statistical significance of different pNANOG quantities between samples was analysed with student's t-test.
Where indicated, significance values between samples were analysed by a paired t test.
Differences between samples were analysed using non parametric Kruskal-Wallis test.
Differences between samples were analysed using an unpaired Student's t-test.
Where indicated, significance values between samples were analysed by paired t test.
