A 1 kHz sampling rate implies 1 ms time interval between samples which is needed for an appropriate software and hardware setup to accomplish protecting relay task within this time interval.
According to the test results with Whatman no.1 paper, RSD was ~2% between measurements as well as between samples, which was found with the standard pH measurement method using an ordinary pH electrode (InLab™ EASY pH electrode, Mettler Toledo).
The relative standard curve method was used to calculate relative mRNA abundance between samples, which was then presented as means+SEM of gene expression from six biological replicates (assayed in duplicate) for each time point.
The bam files were also used to normalize read depth between samples, which was used to compare samples between different pools (Supplementary Table 2).
An empirically setting of statistical thresholds for fold changes between samples, which is most widely used in published studies (we assumed more than two fold up- or downregulation as significant).
It is defined as: (1) D F = V s ∕ U a where Vs is the intra-home variability between samples which is defined as the Coefficient of Variation of LIR of environmental samples (water, dust, soil...) from a child's home.
The latter observation suggests that few cell physiological changes had occurred between the samples which were therefore physiologically more similar to each other at cellular level.
Indeed, Turashvili et al. [ 5] has also reported only 28 genes that were significantly differentially expressed between IDC and ILC samples, which were extracted using laser-dissection, a more recent methodology allowing for precise collection of a given cell population.
Table S2 shows the average log2-fold change in the gene expressions of NFKB2, PTGS2, JUN, MMP1, RUNX1, CEBPA, and FIGF between the relapse and nonrelapse samples, which were 0.02, −0.13, −0.16, 0.7, −0.08, −0.01, and −0.09, respectively.
