Baseline aflatoxin levels and 10× concentrated mushroom samples were quantitated using the Neogen® Reveal® Q+ test kit and AccuScan Pro Reader (Neogen Corp., Lansing, MI) per manufacturer instructions.
The lactose levels in 20 breast milk samples were quantitated by the plasmonic MS and compared to the conventional biochemical method (Fig. 3c, Table S3).
ChIP samples were quantitated by real-time PCR.
All HBoV-positive samples were quantitated by real-time PCR with a second sample aliquot.
Images from these samples were quantitated and the result is plotted in Figure 3(c).
Briefly, protein samples were quantitated by the enhanced BCA protein assay kit (P0010S, Beyotime Institute of Biotechnology, China).
The samples were quantitated by analyzing 25 randomly chosen visual fields per animal.
Protein bands in immunoblots of diluted samples were quantitated in ImageJ.
All DNA samples were quantitated using a Nanovue spectrophotometer (GE LifeSciences, Piscataway, NJ, USA).
Samples were quantitated with a NanoDrop spectrophotometer (Thermo) and stored at −80°C.
