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Comparisons between samples were made after normalization with U6 RNA levels.
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The quantitative comparison between samples was made using CPMG.
Comparisons between these samples were made for the total scores of the WHODASII, MIDAS and PHQ9.
When appropriate, comparisons between two samples were made by Student's t-test or for multiple samples with analysis of variance followed by Tukey's test for assessing individual differences.
Comparisons of transcript levels between the three samples were made using nonparametric tests.
After testing molar concentrations between 0.001 0.5 M, most samples were made using 0.005 M and 0.01 M solutions, as this molar range was found to provide the most uniform and reproducible layers of rods.
Comparisons between control and treated samples were made with a paired Student's t-test.
Comparisons between two groups of independent samples were made with two-tailed, unpaired t tests.
Comparisons between mutant and wild-type control samples were made for fold change estimation using the comparative Ct method, 2−ΔΔCt, where ΔΔCt = ΔCttest gene-ΔCtβ-actin, relative expression was calculated per 1000 molecules of β-actin, 1000/2-ΔΔCt.
Comparisons of the plasma sCD18 levels between groups of unpaired and paired samples were made by using the Student t test in the unpaired and paired modes, respectively.
Protein samples were made anaerobic by cycling between vacuum and argon using a Schlenk line.
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