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The differences of the percentages between samples were compared by paired two-tailed t test.
The differences of the levels between samples were compared using paired two-tailed t test.
The average genetic distance and numbers of unique variations between samples were compared using the Mann-Whitney test.
PCR products were normalized against the housekeeping gene Gapdh, and measurements between samples were compared by cycle threshold (Ct).
Each sample in the Beclin 1 promoter luciferase reporter assay was also run in triplicate and the differences between samples were compared using paired two-tailed t test.
ACTB (β-actin) was used as an internal control, and measurements between samples were compared by the threshold cycle of amplification (CT).
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Equivalence testing is the mathematical determination of the standard deviation of differences in expression values between samples being compared [ 15].
Thus, the outcome of the clustering analysis depends on the method of calculation of the distance between samples being compared.
Identification of differentially-expressed transcripts (i.e. transcripts which differ in abundance between samples being compared) has been conducted for the last ten years on symbiotic interactions between rhizobia and legumes (for review see [ 16]) thanks to improved molecular approaches such as Subtractive Suppression Hybridization (SSH), for example.
The expression level of the KCNJ6 gene was normalized to that of the ACTB gene for each sample, and relative KCNJ6 mRNA expression levels between all samples were compared by setting the lowest expression level among all SMRI samples as 1.
The relative abundance of OTUs with 97%-identity between pair-wise samples or between groups of samples were compared.
More suggestions(15)
between samples was compared
between samples were calculated
between samples were identified
between sites were compared
between years were compared
between samples were quantitated
between formulations were compared
between samples were made
between areas were compared
between variables were compared
between samples were observed
between samples were analysed
between samples were corrected
between samples were performed
between activities were compared
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