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Statistical significance of different pNANOG quantities between samples was analysed with student's t-test.
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Where indicated, significance values between samples were analysed by a paired t test.
Differences between samples were analysed using non parametric Kruskal-Wallis test.
Differences between samples were analysed using an unpaired Student's t-test.
Where indicated, significance values between samples were analysed by paired t test.
Differences between samples were analysed for log10 transformed data by Student's t-test, with significance set at P < 0.05.
Differences between samples were analysed using an unpaired or paired Student's t-test or one-way ANOVA with Bonferroni's adjustment.
The functional difference between each pair of samples was analysed by calculating the distance D2, which can normalize such differences by using the BLAST bit-scores of the best hit.
Concordance of CA IX expression between preoperative biopsies and operative samples was analysed using non-parametric paired-samples test (Wilcoxon signed ranks test or McNemar test).
The significant difference between the means taken from different samples was analysed through independent two-tailed t-tests.
The difference of anti-HBsAb titre between the first and second serum samples was analysed using a non-parametric test: the Spearman rank correlation coefficient.
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