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No significant changes (p > 0.5) were found immediately after thawing between samples stored at subzero temperatures and the 4°C controls.
We also identified differences in the gene expression pattern between samples stored at –80oC and in liquid nitrogen, with 2,193 differentially expressed genes (551 genes with an additional 0.5log2 ratio cut-off), but the FDR stayed relatively high (35%).
This might explain while the qRT-PCR detects differences in mRNA decay between samples stored at ambient temperature and 4 °C in RNA Later that were not found in the sequencing results.
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There was no significant difference in chondrocyte viability between the samples stored at 37 °C and 4 °C (52.02 ± 5.97 % and 57.80 ± 2.71 %, respectively).
However, no difference was observed between the populations in samples stored at 25 and 35 °C after 24 h (Fig. 1).
The similar stiffness and hysteresis properties observed between unfrozen controls and samples stored at -20°C as well as those stored at -80°C may indicate that the freezing rate associated with this storage temperature, when matched with rapid thawing at 37.5°C, may limit constitutional supercooling and thus decrease the opportunity for ice lenses to form.
Sample 121 shows a similar trend between the initial magnetic properties and the samples stored at ambient temperature or 4 °C.
A 2 h 75 g OGTT was performed between 24 and 28 weeks' gestation and samples stored at −80°C for later determination of metabolites.
Then samples stored at −80°C.
Plasma samples stored at −80°C.
There are no major differences in the grain morphology observed between the sample stored at RH 30%% and the fresh one.
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