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Averaging dye-swapped pairs in this manner eliminates systematic dye bias and reduces random variation, allowing the small differences between samples labeled at the two sites to be observed.
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Labelled probes were measured in the spectrophotometer at 550 nm for the samples labelled with Cy3 and at 650 nm for the ones labelled with Cy5.
Second, we observed a clear difference between animals labeled at adulthood or after birth.
Gray lines show the boundaries between assembled linkage groups, labeled at the top of the figure.
We first determined FRET between receptors labelled at the same position.
Collected samples were labeled and stored at -70°C until analysis.
Variability among microarray assays might arise from differences between labeled samples, between the arrays themselves, or between hybridization conditions.
In other words, we keep a large margin between different labeled samples, and in the meanwhile, we use the unlabeled samples to regularize it.
Soil samples were labeled and oven-dried at 80°C for 48 h.
Some factors depend on target sequence, for if there are several mismatches between the samples and the standard sample, the detection of all internal cuts is difficult because the cDNA is only labelled at the 5' and 3' terms.
Qualitatively, the peptide overlap between replicate analyses of the same sample and reverse labeled samples were found to be within 8%.
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