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Further, we investigated whether the γ-secretase components were degraded during the incubation in the activity assay but we found no significant difference in component levels between samples incubated at 37°C for 16 h and non-incubated samples (data not shown).
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The uptake was measured at 48 h after stimulation, and was calculated as the change in MFI between cell samples incubated at 37°C and 4°C.
There was no significant difference in NICD levels between the samples incubated at 37°C and NRC (Figure 2A), indicating that there was no degradation of NICD during incubation.
Again, we found no differences between non-incubated samples and samples incubated at 37°C in the presence of a γ-secretase inhibitor, indicating that there is no degradation of NICD or Aβ in our system.
Kefir samples incubated at 25 °C showed the highest values of viscosity, while the samples incubated at 30 °C exhibited the lowest viscosity.
Kefir samples incubated at 30 °C exhibited the highest flow behaviour index values.
Samples incubated at 90°C for 60 min.
Control samples incubated at 4°C showed no internalized zymosan.
The level of antigen uptake by DCs was expressed as the difference in MFI (ΔMFI) between the cell fractions of the same sample incubated at 37°C and 0°C, respectively.
For NICD, we did not find any differences between samples that were incubated at 37°C in the presence of a γ-secretase inhibitor and the samples that were not incubated at all, indicating that degradation in this case is limited.
Samples were incubated at room temperature for between 30 min and 2 h.
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