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The most striking differences were those observed between samples imaged at t = 72 h.
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Samples were imaged at scan sizes between 1 µm and 10 µm using line scan rates below 2 Hz and 512×512 pixels were collected per image.
Untreated beta-bow samples were imaged at 30 dpf to estimate leaky recombination.
A second batch of samples was imaged at t = 6 h.
All samples were imaged at room temperature.
The samples were imaged at a resolution of × 400 magnification.
All the samples were imaged at room temperature.
To obtain a correlation between NR fluorescence intensity and contact angle, each NR-coated PS sample was imaged at nine locations, which were averaged to represent the fluorescence intensity for that PS sample (three samples per treatment condition).
A 543 nm laser excited the samples and we imaged at 580 700 nm.
The samples were not positively stained and directly imaged at 175 kV.
All samples were taken directly from the kinetics experiments and imaged at 48 h.
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