At 14 dpci, individual clones varied considerably between samples at the same time point (Supplementary Fig S2C and D).
To do this we followed a number of data-driven experiments to establish the effectiveness of 'cross-pollination' between samples of individual types of cyber hate, by mixing samples at the training stage.
Data sets were compared between samples from individuals who carry two rare EHF alleles and samples from individuals with at least one frequent EHF allele.
Factor scores also revealed considerable differences between the two sampling campaigns at individual sampling points.
Over 80% of identified peaks were shared between sampled individuals at a stringent IDR (Additional file 3: Table S2), showing that our approach successfully captured biologically relevant binding events.
Estimates of effects, in the form of changes in population averages over time, are obtained in a way that has been corrected for the correlation between samples from the same individuals at different times and between individuals from the same village.
Researchers studying disease look at how microbial communities differ between samples from healthy and unhealthy individuals (Srinivasan and Fredricks, 2009).
The distance between samples within populations was at least 10 m to increase the likelihood of sampling inter-individual variation within each population.
Differences between samples were also tested at the individual level using a confidence interval test on the individual differences.
Important to this study is therefore not the number of variants with respect to a (arbitrary) reference genome, rather the differences observed between samples collected from the same individual at different times.
Although the difference between gut samples from an individual is smaller than those from different individuals or different body sites, there seems to be no core set of taxa present at all time points.
