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No differences between samples as a function of their curing temperature were observed under SEM.
PARP1 expression was in fact compared between samples as a ratio of PARP1/actin transcript levels.
We define differential transcription of a gene between samples as a difference in the relative abundance of the gene's transcript isoforms in the samples.
However, brain tissue is composed of a variety of cell types such that mRNA expression levels of genes may vary between samples as a result of differences in cell composition of the particular piece of tissue used.
This difference in percentage methylation between array hybridization and pyrosequencing has been noted previously [ 15], suggesting that hybridization to CHARM arrays using either unmethylated-enriched or methylated-enriched DNA may underestimate the relative difference in methylation between samples as a consequence of the fixed dynamic range of microarrays.
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Although far from being impressive we found differences between samples as well.
In general, the random effects model was preferred because of the large variance within and between samples as well as the effects of several very large database studies that may have introduced bias.
Data normalization: in qRT-PCR an endogenous control gene is used to normalize data and control for variability between samples as well as plate, instrument and pipetting differences.
We retained every 10th iteration to reduce autocorrelation between samples as well as for analytic tractability.
Initial reports of differences in exon usage, and splicing between samples as well as quantitative differences among samples are beginning to surface.
Unlike the t-statistic, it is appropriate in cases where the observations take on discrete values and where variation occurs between samples as well as between genes or miRNAs.
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