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Then we compare the average intensity of the same regions between samples and references.
In order to reduce the risk of false positives or false negatives in the final results, SNPs with conflicting genotype call between samples and references are filtered right at the beginning.
Four human genes were included in order to standardise the amount of applied cDNA and to normalise the signals between samples and references.
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Significantly differentially expressed genes (outliers) between sample and reference were selected based on their P-value (a gene with a P-value <0.05 was considered an outlier).
Such a quantitative measure is either an average log fluorescent intensity ratio (between sample and reference DNA) over multiple probes representing the CNP, or the log-intensity ratio of the best probe within the CNP region.
If a proportion of the tumour sample is tetraploid (4n total chromosome complement), which often happens in a number of cancer types, this is not detectable by current SNP-aCGH analyses, due to the necessity to normalize DNA content between sample and reference.
On the other hand, due to the Doppler shift between sample photons and between sample and reference photons, Ihom and Ihet are AC signals.
This section is used to account for a mismatch in optical path length and dispersion between sample and reference arm.
MacCoss et al. (2003) developed a correlation algorithm to detect outlier peptides using fractional changes between sample and reference intensities.
The use of reference sequences that originate from the study sample itself can reduce the potential mismatch of ancestral backgrounds between sample and reference populations, decreasing imputation errors.
Two-color array analyses were used and determination of expression levels among samples was based on differences of ratios between sample and reference.
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