Sentence examples for between samples and calibrators from inspiring English sources

For this purpose, fold differences were calculated from ΔCq between samples and calibrators.

Samples and calibrators were added to a microtiter plate coated with mouse monoclonal antibodies against pig NGAL.

For evaluating the stability of the chosen reference snRNAs among donors as well as samples and calibrators, we have plotted Cq values of samples versus calibrators.

Plasma controls, test samples, and calibrators were added to the antigen-coated 96-well plate.

GUSB and TBP were used as an internal reference in all of the samples and calibrator.

Consequently, variable antibody recognition may be generated between blood samples and internal calibrators among assays.

To control for differences in sampling, DNA preparation, reaction efficiency (the varying PCR efficiencies between patient samples and the calibrator) [ 39] and other variables such as the Ct values for each primer pair [ 40] from 22q11.2 were corrected/normalized using the Ct value of the G6PDH and HEM3 products for the same sample.

The relative quantification (ΔΔCq) was performed by determining the difference between ΔCq sample and ΔCq calibrator.

The correlation analysis revealed high linearity between Cq sample and Cq calibrator possessing a coefficient of determination close to 1 (r2 = 0.9788 and P < 0.0001) and pointing to excellent stability of all reference snRNA.

In the first step of the method, the difference between Ct values of target and housekeeping gene was calculated (ΔCt), whereas in the second step the difference between the ΔCt values of the samples and the calibrator was determined (ΔΔCt).

Thus, RQ reflect the ratio between the expression of target genes in the samples and in the calibrator.