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For each gene i, we calculated the difference between sample means and a two-sample t statistic.
Comparisons between sample means and population means were based on 1 sample t-tests and relationships between variables investigated suing simple correlations.
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However, the difference between sample mean and population norms for HUI3 (diff = 0.14) is nearly 5 times the clinically important difference for the HUI3 overall score [ 8, 31].
Statistical analysis was performed using GraphPad Prism 5 software (GraphPad, San Diego, CA, USA) and comparisons between sample means for static- and OFF-cultured cells were investigated using a Student's unpaired t test.
One estimate of σ2 is based on the variability between the sample means and the variability between each sample data".
This statistical analysis was selected because it tests the hypotheses about the difference between two independent/un-paired population means using the difference between two sample means and does not require that populations have normal distributions.
We assessed PedsQL using effect size by examining the differences in PedsQL scores between our sample means and the means in the reference population.
The comparison of two populations results in a single easily understood statistic the difference between sample means.
However, in population levels of ampicillin resistant bacteria, the minimum difference between sample means to be significant with a likelihood of 80% was 8.9%.
The enhanced two-stage selection (ETSS) is a procedure that takes into account not only the variance of samples but also the difference between sample means when determining the sample sizes.
Differences between sample means were determined using the t test.
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