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Comparisons between sample groups were performed using GeneSpring GX 9.0 software (Silicon Genetics, Redwood City, CA).
Statistically significant differences between sample groups were determined using a Student's t-test or ANOVA where applicable, with a p-value≤0.05 considered to be statistically significant (p≤0.05 = *, p≤0.01 = **, p≤0.001 = ***).
Differences in the frequency of individual CNIs between sample groups were compared using the χ test.
Comparisons of expression levels between sample groups were carried out with lineal regression.
Gene expression differences between sample groups were analyzed using t-test or paired t-test.
Significant differences between sample groups were identified by the 'limma' package [ 16].
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Significant difference between sample groups was identified using the method described by Storey & Tibshirani [ 24].
A repartition difference between sample groups was considered significant when the Kruskal-Wallis nonparametric test and Wilcoxon test gave a P value ≤0.05.
For RT-PCR, the relative difference between sample groups was calculated according to the difference in Ct values using 2−Δ Δ Ct) (31).
Representative variations in the photoluminescence and Raman spectra within flakes and between sample groups are shown in Figure 2. Photoluminescence emission and Raman E2g mode scattering maps were constructed by integrating the respective emission and scattering intensities.
Differential expression between sample groups was assessed using the moderated t-test with p-value correction for multiple testing at a false discovery rate (FDR) threshold of 5% (q < 0.05).
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