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Since differences between replicates were observed within treatments, the data were not pooled for statistical analyses.
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Higher variation between replicates was observed in miRNAs with low expression in both assays.
A high degree of correlation between replicates was observed (r > 0.98).
For three genes, flavanone 3−hydroxylase1 (fht1), liguleless1 (lg1), and yabby15 (yab15), consistent differences in nucleosome organization between mutant and wild type replicates were observed in one or more of the tissues analyzed (Fig. 3).
Moreover, a small variation in the mapping statistics between biological replicates was observed, suggestive of the consistency between two replicates.
No significant variation between samples or replicates was observed.
Furthermore a high correlation between signal intensities representing biological replicates was observed (correlation coefficients r2 = 0.97 0.99).
Overall, a close relationship between technical replicates and among samples harvested at the same time point (biological replicates) was observed, whereas samples from distant time points showed greater variability.
We initially sample the expression for all replicates using the same mean relative expression and variation between replicates as were observed in the Xu et al. data estimates.
Both Mah and Maa were able to infect and replicate within macrophages, however some differences between the subspecies were observed.
Significant differences were observed between replicates for the proportion of positive scores (χ2 = 15.76, df = 2, P<0.001).
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