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No discrepancies between replicates were found in 10 replicated individuals (maximum genotyping error 2.6%).
Out of range values were discarded and 96 99% of the ratios between replicates were found between the two 0.5 2.0 threshold values.
Similar(58)
A varying degree of systematic noise was observed in each of the datasets, however in all cases the relative amount of variation between standard control RNA replicates was found to be greatest at earlier points in the sample-preparation workflow.
Concentration point RSD values (six replicates) was found to be less than 2%.
In S2, in both replicates, perithecia were found all over the plate and not just at the line of contact between the different mycelia at the 9th AG.
In P6 utricles, the first replicating SCs were found at 2 DIV (EdU pulse between days 1 and 2).
However the replicate UHRR samples were found to slightly improve the consensus between the results of the duplicate experiments when used in conjunction with the ComBat correction.
Only the fusion events in common between replicates were retained.
In addition, the correlation coefficients (R) between replicates were calculated.
High Pearson correlation r values were found between technical replicates even when using different concentrations of RNA for amplification (Average 0.993; Range {0.977 to 0.997}).
No significant differences were found between independent replicates of any particular GM line, and the different lines harboring the same bp100der transgene gave similar values for all studied parameters (one-way ANOVA P values above 0.05).
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