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Each forebrain was treated as a biological replicate in Cuffdiff and therefore variation between replicates was considered when assigning a p-value.
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Therefore, genomic coverage as well as overall error rate and extent of variability between technical replicates were considered.
Among these, only genes which had the same expression profile between the two biological replicates were considered.
Only proteins detected in at least 3 biological replicates were considered for downstream analysis of differentially regulated proteins between rhizomes (tip and zone) and root tissues.
7 fields per sample and 2 replicates were considered.
Three replicates were considered.
Three replicates were considered in the experiment.
Only those spots with at least two replicates were considered.
Three biological replicates were considered for each experiment.
Only the spots with at least three replicates were considered.
Match between run options within a 2 min time window was enabled for constitutive fractions and only proteins identified in two out of three biological replicates were considered.
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