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The associations between protein expression values and pathological stage were assessed using the χ-test.
For determination of the correlation of the effect of the initial dose with response to chemotherapy, the differences between protein expression values of time T2 and T1 were assessed for each spot.
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The qRT-PCR data were expressed relative to the expression of β-actin using the 2-△△Ct method, an independent-sample t-test available in SPSS software (Version 16.0, SPSS Inc .. Person correlation was used to access the relativity between transcript and protein expression values [ 44].
Hence, Mann-Whitney and Kruskal-Wallis non-parametric tests were used to compare median protein expression values between two and three groups, respectively.
Quantification of protein expression for all blots was performed using Image J densitometry software (https://imagej.nih.gov/ij) with protein expression values normalised to the loading control (GAPDH).
The ratios between the signals of the protein of interest and β-actin were calculated to determine the relative protein expression values.
#PRDX1 protein expression levels are stratified by the median protein expression value.
Of the 46 radiation-responsive proteins, four up-regulated proteins FDXR, BAX, DDB2 and ACTN1 were considered as the top 4 candidates based on large fold changes >2 and a strong correlation between protein expression and irradiated dose (correlation coefficient >0.7; p value < 0.05).
P values relate to the differences between protein expression and the variables.
In this work we have examined the relationship between protein expression and gene sequence characteristics, using two different proteins of commercial value for which expression levels were limiting.
To test the dose dependent response, the correlation between protein expression and irradiated dose was evaluated for all 46 radiation responsive proteins.
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