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In May 2010, crosses within and between populations were produced, using 40 parental individuals (20 females and 20 males) from each of the two populations.
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To clarify whether these differences in phenotype between the CD44high and CD44low populations were produced by CD44s, we overexpressed CD44s in PLC cells with low CD44s expression, or depleted CD44s expression in HLE cells with high CD44s expression, and examined the effect on sphere-forming ability.
By making sexual crosses between resistant and susceptible Solanum genotypes, experimental (segregating) populations were produced.
Mixed BC1/F2 populations were produced in the greenhouse.
120 progenies per population were produced.
After checking that meat quality traits differed between the two lines, an F2 population was produced and used for QTL detection, with 108 microsatellite markers.
This population was produced from year 2000 to 2006.
Between July and August, laboratory-bred F1 families were produced for each population (20 families per population) using a modified North Carolina II breeding design (five replicates of crosses between two dams x two sires i.e. four half-sibling families).
The mapping populations of 190 F8, F8 and F9 RILs were produced by single seed descent (SSD) from an F2 population of a cross between DN422 and KY131.
Upon differentiation, populations enriched for neurons (hNPC-Ns) were produced.
Grid cells were selected with population greater than zero in each year, and pairwise correlations (Pearson's 'R') were produced between all combinations of years.
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