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The significance of differences between populations of data were assessed according to the Student's t-test with a level of significance of at least P<0.05 (alpha conventionally equal to 0.05).
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Significant differences between different populations of data were tested with Student t-test for unpaired observations.
Comparisons between two populations of data were made using the Student's unpaired t-test with a confidence limit for significance set at 0.05 or less.
ANOVA was used to test statistical significance between different populations of data.
FRET-FLIM experiments were repeated at least twice, and ANOVA was used to test statistical significance between different populations of data.
A previous FST scan based on nuclear transcriptome data, between populations of Ma .balthica spanning the same two secondary contact zones as considered here, detected strong population structure at several genes involved in the ATP synthase and NADH deshydrogenase complexes [ 24].
There are huge differences between populations of humans and ants.
This suggests that the transition between PE and HFA is represented by one single population of data, likely reflecting a unique process characterized by a continuum of thermal energy release.
We found that CaSA was able to quantify significant and consistent differences between the two populations of spiking data sets.
Between-population divergence of the Pvama1 data presented here and previously published Pvama1 data for Domain I (DI) from global sites [15], [33], [34], [35] was determined using the θ-estimator of Wright's fixation index (FST) to determine the relative contribution of the differences observed between each population to the overall diversity seen using Arlequin v.3.11 software [36].
There was no difference in offspring production between the populations (all data pooled, medians of 79&6969 for UK and Greek females respectively; Mann Whitney U-test, W = 72429, p = 0.080).
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