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We identified 2308 genes that differed in expression between the sexes and 2474 genes that differed in expression between populations at a false discovery rate of 5%.
The differentiation between populations at a given SNP position is then evaluated from the estimated cluster frequencies at this position using statistics related to a multi-allelic FST, but it accounts for population structure through the previously estimated kinship matrix.
First, NGS makes it possible to study the genetic variation within and between populations at a level that is more representative for the entire genome, while using tens of thousands (instead of tens or hundreds) of markers.
The long disagreement over species concepts is the best demonstration of such a continuum between populations, at a proximal scale, to the ultimate extreme of reproductively isolated species [ 2].
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Global F-statistics indicate that there is highly significant population differentiation between populations at all microsatellite loci.
The Fst statistic captures the difference in allele frequency between populations at any given SNP and ranges from 0 (no differentiation) to 1 (fixed difference between populations).
It provides a way to manage and share risk between populations at local and global scales, via public, private and mutual mechanisms, enabling us to care for one another and thereby underpin our human dignity.
The combined effect of these selective processes is here demonstrated in an extreme case to generate isolation barriers between populations at high altitudes.
Therefore, respiratory performance is expected to vary between populations at different latitudes because they experience different thermal environments.
Critically, for conservation planning, the lack of connectivity between populations at different depths will have consequences for the fate of species and populations affected by environmental change.
We used FDist2 [27] implemented in Lositan [39] to further assess the effect of any selection on the variation between populations at the MHC or microsatellite loci.
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