syringae B728a, only the first two contain plasmids, with two plasmid types per genome [119], [120], [121].
Plasmids with two or more minireplicons are significant larger than plasmids with only one minireplicon.
Therefore, plasmids with two cer sites possibly mimic multimers, decelerate cell division and facilitate accumulation of plasmid DNA.
Among the 771 plasmids with putative minireplicons (Additional file 5: Table S4), one third of the plasmids have two or more minireplicons and plasmids with two or more minireplicons are larger than those with only one minireplicon.
The anthrax bacterium spends most of its time in the soil but has moved up the food chain from scavenger to killer by having acquired its plasmid with the three toxins.
In case of simultaneous transfection with two separate plasmids, 30 μg to 60 μg of each plasmid DNA were used.
This plasmid was co-transfected with three auxiliary plasmids into HEK 293 T cells using the polyethyleneimine method.
Pps were produced by transfecting 293T cells with four plasmids: an HA expression plasmid, an NA expression plasmid, a Gag-pol-encoding plasmid, and a reporter plasmid.
The five plasmids with promoters from JPU strain were named Y2JCYC, Y2JTEF, Y2JPGI, Y2JPGK and Y2JADH, and the five plasmids with promoters from S288C strain were named Y2SCYC, Y2STEF, Y2SPGI, Y2SPGK and Y2SADH.
Experiments were conducted with two types of plasmids.
