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As we describe in detail above in response to reviewer point 3 above about the link between phosphorylation and dynamics, in the initial version of the manuscript we went too far in trying to firm up what was an admittedly speculative link.
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It is likely that these complex dynamics may also emerge from crosstalk between phosphorylation and ubiquitination.
Conversely, activation of phosphatases (shifting of the equilibrium between phosphorylation and dephosphorylation reaction towards dephosphorylation) should result in blockade of caveolae dynamics.
Protein kinases were significantly enriched among SUMOylation substrates, suggesting crosstalk between phosphorylation and SUMOylation.
Related to histone phosphorylation, a crosstalk between phosphorylation and acetylation of neighboring residues has been detected.
This may suggest additive roles for multiple phosphorylated residues, or may suggest the connection between phosphorylation, dynamics and function is more complex.
Furthermore, we evidenced a causative relationship between phosphorylation dynamics and the progression of spore revival by demonstrating that spores bearing mutations at specific phosphorylation sites within proteins, representative of key biological processes, exhibit aberrant spore revival phenotypes.
Of the proteins listed in Table 1, very few have been studied carefully regarding the quantitative connection between multisite phosphorylation and degradation dynamics.
Therefore, lower eukaryotes provide an excellent model to study a causal link between histone phosphorylation and chromosome dynamics in vivo.
Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg.
Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in theegg.
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