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Author's response: There are no correlation was observed between pathway position and gene duplicability.
Additionally, we conducted a modified Monte Carlo test controlling for the association between pathway position and selective constraint.
However, removing the influence of the association between pathway position and selective constraints yields equivalent results (supplementary table S18, Supplementary Material online).
Moreover, this analysis showed a significant association between pathway position and ω and dN for data set 3 when O. anatinus was not considered.
The results of the Monte Carlo simulation analysis also support the overall association between pathway position and selective constraint (supplementary table S12, Supplementary Material online).
However, some studies have failed to detect a correlation between pathway position and sequence divergence (Yang et al. 2009; Jovelin and Phillips 2011).
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Many reports have demonstrated the existence of a positive correlation between gene pathway position and nucleotide substitution rate.
In analysis of the correlation between the pathway position and dN/ dS, the authors exclude genes in position 7 and 8 of HOG pathway.
We have previously shown that in the Drosophila insulin/target of rapamycin (TOR) signal transduction pathway there is a correlation between the pathway position and the strength of purifying selection, with the downstream genes being most constrained.
Furthermore, after removing O. anatinus sequences from the analysis, the correlation between the pathway position and the overall dN values is significantly negative for data set 2 (table 2).
A correlation between the pathway position and the strength of purifying selection has also been observed in other pathways, including the anthocyanin, isoprene, terpenoid, and carotenoid biosynthetic pathways in plants (Rausher et al. 1999; Sharkey et al. 2005; Livingstone and Anderson 2009; Ramsay et al. 2009) and the Ras signal transduction pathway in Drosophila (Riley et al. 2003).
More suggestions(15)
between pathway status and
between pathway architecture and
between pathway adherence and
between pathway involvement and
between load position and
between body position and
between pole position and
between landscape position and
between intron position and
between class position and
between gene position and
between wound position and
between network position and
between birth position and
between lithotomy position and
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