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Thus, enhanced LOX activity was detectable in cellular homogenates as well as in the culture supernatant during HMEC aging between passage 12 up to passage 16.
For all experiments, cells between passage 3 and 8 were used.
In all the experiments, cells were used between passage 4 and passage 6. Immortalized human keratinocytes (HaCaT) were grown in the same conditions.
A431 cells were obtained from the Ludwigshafen Institute, cells were grown between passage 3 6 in vitro in RPMI 1640 media with 10% FBS.
The French high court examined 105 cases between passage of the law in 1972 and 2012.
If comprehensive health care reform is passed this spring, it will rapidly increase in popularity between passage and next fall's elections.
HUVEC were used between passage 3 and 6.
All fibroblasts were used between passage 3 and 6.
All experiments were performed between passage P5 and P15 after isolation to avoid premature replicative senescence.
For our experiments, cell populations between passage 3 and 5 were used.
Chromosome banding analysis was performed between passage 3 to 4 by standard methods.
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