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The difference in filopodia numbers was significant between paired data sets showing low and high red-to-green pixel intensity ratios (ts = 4.13, p = 0.00029), highly significant between data sets showing low and high red pixel intensity (ts = 5.72, p = 0.000006,) and not significant between data sets showing low and high green pixel intensity (ts = 1.29, p = 0.207).
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Paired data sets from TBW estimates were compared using paired t-tests.
Data are expressed as mean ± s.e.m. and paired data sets compared using two-tailed t-tests.
For comparison of samples, the Mann-Whitney test was used for non-paired data sets, including comparison between different experimental days, and the Wilcoxon signed rank test was used for paired data sets, including comparison between materials on the same experimental day.
Overall, 54-paired data sets of PaCO2 and EtCO2 from 11 patients (seven men/four women) could be recorded and were included in the analysis.
The root-mean-square (RMS) differences between paired data were used as the measure of reliability.
Figure 1A shows the standard deviation of the log ratio of the 4 paired cDNA data sets (A-D).
However, paired-end data sets have more difficulty detecting structural variation in certain genomic regions, such as rearrangements flanked by transposable elements.
These paired-end data sets were then aligned to the melon reference genome to produce bam files as described in the Methods section.
Coverage from the paired end data set was deep enough to estimate nuwt copy number.
Examining the paired end data set in more detail showed that BWA-detected nuwts in B. malayi originated from 116 wBm genes (Additional file 1).
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