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This interpretation is strengthened by the recent demonstration that prognostic gene expression signatures pre-exist between normal tissues of animals of high- or low-metastatic genotypes [ 27].
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To further investigate whether differences found in protein levels of CYPs between normal tissue of patients with adenoma and disease-free controls are related to the mRNA expression of CYPs, protein levels of CYP2C8, CYP3A4, and CYP3A5 of subjects with detectable mRNA expression were compared to those with undetectable mRNA expression of the respective CYP.
It also appears capable of differentiating between normal tissues classified by phase of the menstrual cycle, although it was not able to distinguish between menstrual and proliferative tissues.
The expression level of miR-224 increased between normal tissues and the early stage of CRC but did not show significant differential expression between the early and late stages in a global CRC specimen miRNA analysis [ 12].
MRI has been shown to have better differentiation between normal tissues and many different types of tumors.
By comparing the color tone of autofluorescence between normal tissues and malignant lesions, we were able to devise a highly accurate method of localizing malignant lesions.
As indicated in Additional file 1: Figures S1C and D, PCA indicates that miRNA expressions between tumors vs. normal tissues of formalin-fixed paraffin-embedded tissues (FFPE) were better separated than fresh-frozen (FF) samples.
Second, we investigated the extent and types of allelic imbalance (AI) observed between the tumor and normal tissues of the three patients.
Differential gene expression values, between the tumor and normal tissues of a particular patient, were compared with the corresponding values from RNA-Seq data (Table S2).
The complexity makes the identification of the most important differences between the tumour and normal tissues of the asbestos-exposed using genome-wide gene expression difficult.
As shown in Figure 3A, their mRNA expressions were significantly different between cancer and match normal tissues of 22 patients with UTUC (***P <0.001, paired t-tests).
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