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The TMA method allowed a comparison between multiple cell pellets or tissue specimens on one microscope slide.
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Total lipid extracts were obtained by multiple extractions of the lyophilized cell pellets (5 × 10 cells) with chloroform/methanol (2 1 and 1 2 (v/v) at 4°C.
Comparison between the cell pellet and the cell-free soluble extracts revealed the formation of inclusion bodies.
Cell volumes were determined by the distribution of H-labelled H2O (0.55 mCi/l) and C-labelled inulin (0.14 mCi/l) between the cell pellet and the supernatant.
The cell pellets were washed multiple times with Hank's Balanced Salt Solution (HBSS), re-suspended in HBSS, and red blood cells were removed by density centrifugation with Lympholyte H (Cedarlane, Burlington, ON), according to the manufacturer's protocol.
Cell pellets were cleared in xylene and washed multiple times in molten paraffin.
Cell pellets are placed between the two ends of AMF.
MR imaging of cell pellets revealed a large contrast between specific and control incubations.
Conclusion: It is feasible to conduct multiple molecular analyses at the deoxyribonucleic acid, ribonucleic acid, and protein levels of the cervicovaginal cell pellets from the residual fluid-based Papanicolaou cytologic specimen.
We observed generally excellent agreement between EGFR staining in CTCs and EGFR levels determined by IHC on cell pellets.
Pearson's correlation analysis was conducted to assess the potential associations between changes in relative mitochondrial [Co]Cbl levels and [C] propionate incorporation into TCA-precipitated cell pellets.
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