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In this study, we focused on the relationship between microwell size and mouse EB properties.
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Therefore, we evaluated the relationship between cell capture efficiency, the microwell size, the cell trapping time and the initial cell density.
These results indicate that the difference in microwell size affects the proliferation and differentiation of embryonic stem cells, and that microwell culture is a promising technique to control EB properties.
Remarkably, in softer microwells, cytoskeleton assembly was diminished for the same range of microwell sizes (∼20 kPa; Fig. 3A, S4).
Thus, there was a minor systematic difference between microwell and PBM-derived matrices, but its magnitude was so small that it is unlikely to affect downstream analyses.
Choose between muscle size and muscle tone.
Find a balance between size and quality.
Here, we used engineered concave microwell arrays to regulate the size and shape of embryoid bodies (EBs)—cell aggregate intermediates derived from ES cells.
This is evidenced in that single cell trapping efficiency is dependent on cell type and size, and on microwell geometry [14], effectively selecting cells of a specific size from within the overall population.
This work describes a porous microwell scaffold with a well-defined pore size and spacing designed to guarantee islet retention at an extrahepatic transplantation site and facilitate islet revascularization.
HepG2 cells seeded in the microwells formed 3D spheroids with controlled sizes and shapes depending upon the dimensions of the microwells.
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between birth size and
between intron size and
between body size and
between sperm size and
between pore size and
between population size and
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