However, a high level of concordance (r = 0.97) was observed between the microarray results and the real-time RT-PCR data (Figure 16), which confirms the reliability of the data produced in this study.
The relatively high level of correlation between our microarray results and k-PCR data supports the validity of this microarray platform in revealing gene expression changes.
The relatively high level of correlation (0.85) between our microarray results and k-PCR data supports the validity of our microarray analysis in revealing gene expression changes.
Thus, the concordance between the microarray results and the RT-qPCR results was complete, supporting the statistical approach that was adopted in this study.
Overall, we found a favorable correlation between the microarray results and both quantitative and semi-quantitative PCR analysis for daughter and non-daughter primary and MM cell lines.
In all four groups there was a strong positive correlation (correlation coefficient r > 0.968) between the microarray results and the qRT-PCR, supporting the validity of the microarray method.
The correlation between the microarray results, and those obtained by qRT-PCR was assessed by calculating the Pearson's product moment correlation coefficient [ 41, 42] at replicate level (Table 2).
The correlations between Agilent miRNA microarray results and qPCR results are generally excellent, as are the correlations between different total RNA prep methods.
Pairwise comparisons were made between individual mutant microarray results and controls.
