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Other factors could contribute to greater differences between mice within a cage.
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Each mouse was assigned either the left or right lever to the active lever, and the side of the active lever was counterbalanced between mice within each genotype.
While there was great variability between mice within each recording session, and there was considerable variability for each mouse between sessions, the group average was not statistically different across sessions (p = 0.72, repeated measures ANOVA).
We decomposed each gene profile into a between-mouse profile and a within-mouse profile.
We used random effects ANOVA to decompose total variance into between-mouse and within-mouse variance components.
The terms σ bg 2 and σ wg 2 are the between-mouse and within-mouse variance components in this model.
All experiments were conducted with litter matched wild type (WT) and GluN2B KI mice of mixed sex (evenly distributed gender ratios) and between 2 and 4 months of age; mice within a cohort were not more than 3 weeks apart.
Asterisk indicates significant differences between WT and VLCAD−/− mice within an experimental set.
Values are represented as mean ± SEM (n = 5-6).* indicates significant differences between WT and VLCAD-/ mice within an experimental set.
Our sampling design enabled us to partition the variance for each gene into within-mouse and between-mouse components, with a division line that corresponds to the step of splitting tissues.
Following immunization of mice using the OVA-conjugates, AM1-OVA was found to elicit similar titers to the conjugate from the first round of vaccinations as well as a similar pattern of variation between the mice within the group, as expected given that both conjugates had similar hapten densities (study 1: 11.4 copies, titer of 33 518; study 2: 10.6 copies, titer of 54 754).
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